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Preliminary Study on Sonication-assisted Agrobacterium-mediated Transformation of Houttuynia cordata Thunb
Author(s): 
Pages: 9-11
Year: Issue:  4
Journal: Acta Agriculturae Jiangxi

Keyword:  超声波辅助农杆菌介导鱼腥草遗传转化GUS瞬时表达;
Abstract: 应用超声波直接转化法、农杆菌介导转化法、恒定频率的超声波仪处理植物15 min后再用农杆菌介导法侵染植物和在农杆菌侵染过程中辅助以超声波处理10 s 4种方法对鱼腥草进行转基因研究,结果显示:上述4种转化法对gus基因最大瞬时表达率依次为47%、60%、80%和75%.结论:用恒定频率的超声波仪处理植物15 min后再用农杆菌介导法侵染植物和在农杆菌侵染过程中辅助以超声波处理10 s两种转化法gu...
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