Author(s): LAN Jiongcai, SHI Ning, ZHENG Shirong, CHEN Qiang, LIU Hongyu, WU Dalin, CAO Qiong, YANG Chongli
Pages: 228-229
Year: 1999 Issue:  5
Journal: CHINESE JOURNAL OF IMMNOLOGY
Keyword:  可溶性人类白细胞抗原(sHLA); 酶联免疫吸附试验(ELISA); 定量检测;
Abstract: 目的:sHLA-Ⅰ可预测器官移植排斥反应及监控某些疾病.为了介绍sHLA-Ⅰ类抗原定量检测技术及中国人sHLA-Ⅰ正常值.方法:采用ELISA双抗夹心法,以W6/32 mAb包被酶标板,加被检血清,再加β2m-HRP及底物显色.以不同浓度sHLA-Ⅰ标准品的结果绘出标准曲线并测定113例正常人sHLA-Ⅰ含量.结果:ELISA法可以准确定量测定血清中sHLA-Ⅰ,中国人sHLA-Ⅰ正常值为789.27±506.32 ng/ml.结论:ELISA法检测sHLA-Ⅰ具有特异、敏感及重复性.