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Issue:
Induction of cell cycle arrest in bladder cancer RT4 cells by capsaicin
Author(s):
LI Qin
,
WANG Xinghuan
,
YANG Zhonghua
,
WANG Huaipeng
,
YANG Zhiwei
,
LI Shiwen
,
ZHENG Xinmin
Pages:
1230
-
1233
Year:
2010
Issue:
18
Journal:
National Medical Journal of China
Keyword:
Bladder neoplasms
;
Cell cycle
;
Capsaicin
;
Abstract:
目的 了解辣椒素对膀胱癌RT4细胞生长的影响及可能机制.方法 采用细胞计数kit-8(CCK-8)试剂盒观察辣椒素(50、100、150、200、250μmol/L)对膀胱癌RT4细胞生长的影响,以辣椒素(0 μmol/L)为对照组;流式细胞术检测细胞周期和凋亡;逆转录-聚合酶链式反应(RT-PCR)和免疫荧光检测瞬时受体电位香草酸亚型1(TRPV1)的表达;Western印迹检测细胞周期蛋白P5...
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