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Cloning and sequence analysis of human lactoferrin and construction of the recombinant retroviral vector of PLNCX2-hLF
Pages: 700-703
Year: Issue:  4
Journal: Journal of Southwest University for Nationalities(Natrual Science Edition)

Abstract: 从正常人外周血中性粒白细胞中提取总RNA,用RT-PCR的方法扩增人乳铁蛋白(hLF) cDNA,将其克隆到pMD18-T Simple载体上并测序.用限制性内切酶XhoⅠ和Hind Ⅲ将目的片段与逆转录病毒载体PLNCX2进行双酶切,并用T4连接酶进行连接;然后转化到top10菌中,再次进行DNA序列测定.前后两次序列测定结果一致,没有突变,全长为2,136bp,说明所克隆的为hLF基因的cDN...
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