Author(s): CHENG Yongqian, WANG Lin, CHENG Jun, LIU Zuo, XU Dongping, ZHONG Yanwei, LI Xiaodong, DAI Jiuzeng
Pages: 68-71
Year: 2007 Issue:  2
Journal: Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version)
Abstract: 目的 明确丙型肝炎病毒非结构蛋白4A(HCV NS4A)与钙离子信号调节亲环素配体(CAML)相互结合的具体位点.方法 构建CAML不同剪接体及缺失突变体的酵母表达载体,转化入酵母Y187菌株,分别与转化了HCV NS4A的酵母AH109菌株进行配合,在含X-α-gal的酵母四缺培养基上培养观察.结果 转化了CAML不同剪接体及CAML缺失突变体pGADT7/CAML-1-211,pGADT7/C...