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Research on Orthogonal Optimization of ISSR-PCR System in Chinese Jujube
Author(s): 
Pages: 116-120
Year: Issue:  2
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  枣树ISSR-PCR反应体系正交优化;
Abstract: 为建立适用于枣树ISSR-PCR的最佳反应体系,本研究针对Mg2+、dNTPs、Taq DNA聚合酶和引物共4个因素分别设置4个浓度水平进行正交优化设计.采用极差、方差和多重比较的方法进行分析,以期获得最佳优化体系.研究结果表明:20 μL ISSR-PCR反应体系中4个因素的最佳浓度分别为2.0 mmol/L Mg2+ 、0.2 mmol/L dNTPs、0.05 U/μL Taq DNA聚合酶...
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