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Issue:
Experimental study of urease activity in marine microalgae
Author(s):
XU Ning
,
SUN Shugang
,
DUAN Shunshan
,
LI Aifen
,
ZHANG Chengwu
Pages:
689
-
693
Year:
2010
Issue:
5
Journal:
China Environmental Science
Keyword:
microalgae
;
urease activity
;
urea
;
phytoplankton
;
red tide
;
Abstract:
为进一步了解海洋微藻对尿素的吸收和利用机制,揭示水体尿素浓度升高对近海浮游植物群落演替的潜在影响及对近海有害藻类水华(HABs)形成的促进作用.对海洋微藻脲酶活性的测定方法进行了研究.以典型赤潮藻东海原甲藻为实验材料.在Peers方法的基础上,探讨了失活处理时间、提取液pH值、酶促反应温度以及微藻生长阶段对脲酶活性的影响.结果表明,失活处理时间、提取液pH值、酶促反应温度以及微藻所处的生长阶段都对...
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