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Issue:
Effect of DEHP on IL-2 in EL4 cell
Author(s):
GUO Li
,
PEI Xiucong
,
ZHANG Yumin
,
DUAN Zhiwen
,
MA Mingyue
Pages:
253
-
254
Year:
2010
Issue:
2
Journal:
Chinese Journal of Public Health
Keyword:
di-2-ethylhexyl phthalate (DEHP)
;
mouse T-cell lymphoma (EL4)
;
intedeukin-2 (IL-2)
;
Abstract:
目的 通过邻苯二甲酸二(2-乙基)己酯(DEHP)影响小鼠淋巴瘤细胞(EL4)分泌白细胞介素-2(IL-2)蛋白影响机制研究,探讨DEHP对辅助性T细胞1(Th1)型细胞因子影响.方法 不同浓度DEHP染毒以25 ng/mL佛波酯(PMA)+1μg/mL离子霉素(Ion)激活的EL4细胞,并用钙调神经磷酸酶抑制剂FK506进行干预;实验6 h用逆转录聚合酶链反应(RT-PCR)检测活化T细胞核因子...
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