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Effect of DEHP on IL-2 in EL4 cell
Author(s): 
Pages: 253-254
Year: Issue:  2
Journal: Chinese Journal of Public Health

Keyword:  di-2-ethylhexyl phthalate (DEHP)mouse T-cell lymphoma (EL4)intedeukin-2 (IL-2);
Abstract: 目的 通过邻苯二甲酸二(2-乙基)己酯(DEHP)影响小鼠淋巴瘤细胞(EL4)分泌白细胞介素-2(IL-2)蛋白影响机制研究,探讨DEHP对辅助性T细胞1(Th1)型细胞因子影响.方法 不同浓度DEHP染毒以25 ng/mL佛波酯(PMA)+1μg/mL离子霉素(Ion)激活的EL4细胞,并用钙调神经磷酸酶抑制剂FK506进行干预;实验6 h用逆转录聚合酶链反应(RT-PCR)检测活化T细胞核因子...
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