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Establishment of Cell Line Stably Transfected with the Constructed Plasmid of Mus Musculus FcγRⅡ
Author(s): 
Pages: 133-135
Year: Issue:  2
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  小鼠IgG受体(FcγRII)细胞系玫瑰花环稳定转染;
Abstract: 为建立小鼠IgGFc受体(moFcγRII)稳定表达的哺乳动物细胞系,PCR克隆小鼠moFcγRII片段,构建真核表达载体pcDNAmoFcγRII.转染COS-7细胞后通过G418抗性筛选和连续克隆,获得了在COS-7细胞表面稳定表达FcγRII的真核细胞系.玫瑰花环试验结果显示其阳性率在90%以上.
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