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Cloning of CsCAB Gene from Navel Orange and Construction of Its Plant Expression Vectors
Author(s): 
Pages: 40-43
Year: Issue:  2
Journal: Acta Agriculturae Boreali-Sinica

Abstract: 从脐橙果皮褐变相关基因的cDNA抑制差减文库中,获得了一个CAB基因家族的同源EST序列,通过快速扩增cDNA末端(RACE)方法成功克隆得到984 bp的CsCAB全长基因(GenBank登录号EF010854).序列分析结果表明,CsCAB包含一个621 bp的开放阅读框(ORF),编码207个氨基酸;CsCAB蛋白与钙结合蛋白具有很高的同源性,且具有钙结合蛋白的保守结构域EF-hand.并构...
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