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Immunoscreening and Sequencely Organization of the Helicoverpa armigera cDNA Expression Library for cDNA Clones
Author(s): 
Pages: 18-22
Year: Issue:  2
Journal: Acta Agriculturae Boreali-Sinica

Abstract: 昆虫围食膜是保护其中肠的一道物理屏障,破坏围食膜就可能使其中肠处于不正常的生理状态而最终导致死亡.本研究提取棉铃虫Helicoverpa armigera(Hübner)围食膜蛋白,成功制备棉铃虫围食膜蛋白多克隆抗体,并对棉铃虫中肠cDNA表达文库进行免疫筛选,共得到385个阳性克隆.对其中26个克隆进行鉴定并测序分析,结果表明19个克隆为肠粘蛋白.过碘酸-希夫试剂(PAS)显色法检测棉铃虫围食膜...
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