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FTA-PCR METHOD FOR DETECTION OF CYCLOSPORA CAYETANENSIS IN JUICE
Author(s): 
Pages: 59-63
Year: Issue:  1
Journal: Chinese Journal of Veterinary Parasitology

Abstract: 为建立一种快速检测果汁饮料中卡耶他环孢子虫的方法,采用FTA卡片法提取果汁饮料中的卡耶他环孢子虫DNA,利用特异性引物组FIE/R2B、CC719/CRP999进行巢式PCR.以柔嫩艾美耳球虫、贝氏隐孢子虫等作为对照,结果显示,除卡耶他环孢子虫外,均无特异性条带.用卡耶他环孢子虫卵囊进行巢式PCR敏感性试验,结果显示敏感度最高达1.0×100个/mL.可见,FTA-PCR方法特异、敏感,能用于新鲜...
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