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A Protocol for Rapid Isolation of Total RNA from Plant Tissues
Author(s): 
Pages: 140-144
Year: Issue:  2
Journal: Acta Agriculturae Boreali-Sinica

Abstract: 为验证NaAc/无水乙醇沉淀法提取植物总RNA的质量,本研究对小麦不同组织及其他不同种属植物花的总RNA进行提取和检验,结果表明,该方法提取的植物总RNA完整性好、纯度高.以Tubulin为参照对其余4个持家基因GAPDH、Actin、Rubisco和Ubiquitin的表达情况进行分析.扩增结果表明,选定的各持家基因在叶锈菌侵染不同时间的小麦叶片中的表达量并不一致,且各基因在小麦叶片中的丰度也存...
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