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Study on the Interaction Mode between the Er (Ⅲ) (HE)3 and DNA by Spectrometry
Author(s): 
Pages: 19-23
Year: Issue:  1
Journal: Journal of Southwest University of Science and Technology

Keyword:  Er(Ⅲ)(HE)3配合物鲱鱼精DNA荧光猝灭分子探针;
Abstract: 以Tris-HCl缓冲溶液作为研究介质,在pH=6.75的环境下,应用电子吸收光谱和荧光光谱法探讨了苏木素(HE)和铒(Ⅲ)形成的配合物Er(Ⅲ)(HE),与鲱鱼精DNA的作用方式,并用摩尔比法和双倒数法求得金属与配体的结合比为1:3,Er(Ⅲ)(HE),配合物与鲱鱼精DNA的结合常数为Kθ25℃=1.87×104L/mol.以吖啶橙(AO)作为分子探针探测了Er(Ⅲ)(HE),配合物与DNA的作...
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