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Effects of the calpain system expression on atrial structural remodeling in canine with atrial fibrillation
Author(s): MA Xiao, ZHANG Wei, ZHONG Ming, LI Li, MIAO Ya, SUN Hui, ZHANG Yun
Pages: 1883-
1886
Year: 2007
Issue:
10
Journal: CHINESE JOURNAL OF PATHOPHYSIOLOGY
Keyword: 心房颤动; 卡配因; 犬;
Abstract: 目的:探讨慢性房颤犬心房肌钙激活蛋白酶(calpain)系统表达水平的改变及其与心房重构的相关性.方法:17只杂种犬随机分为心房颤动组(11只)和对照组(6只),于起搏前后均进行经胸超声心动图检查,测量舒张期左房内径.房颤组经颈外静脉将电极置入右心耳快速起搏(400 beats/min)8周复制房颤模型,开胸取心房组织,测定心房肌Ca2+浓度,用荧光实时定量PCR和Western blotting技术检测calpain及其抑制剂calpastatin mRNA和蛋白的表达量.结果:房颤组心房肌Ca2+ 浓度升高,左房内径显著大于起搏前及对照组(P<0.05),房颤组和对照组比较犬心房肌calpain Ⅰ、calpain ⅡmRNA表达无显著差异(P>0.05),房颤组calpastatin mRNA表达明显高于对照组(P<0.05);房颤组calpain Ⅰ、calpain Ⅱ蛋白表达明显高于对照组(P<0.05),calpastatin蛋白表达明显低于对照组(P<0.05).Calpain Ⅰ、calpainⅡ蛋白表达水平与左房内径呈显著正相关(r=0.53,r=0.67,P<0.05),calpastatin蛋白表达水平与左房内径呈显著负相关(r=-0.74,P<0.05).结论:房颤所引起calpain系统的蛋白表达改变,使calpain/calpastatin系统相互间作用失衡,造成多种蛋白被降解可能是心房重构的重要机制.
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