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Chemiluminescence Immunoassay for Chloramphenicol
Author(s): 
Pages: 185-195
Year: Issue:  1
Journal: China Nuclear Science and Technology Report

Keyword:  氯霉素 化学发光免疫分析 辣根过氧化物酶;
Abstract: 采用化学发光免疫分析(CLIA)技术检测动物性食品中的氯霉素(CAP)残留量。将兔抗氯霉素多抗吸附在聚苯乙烯微孔板上制成固相抗体,在氯霉素上连接辣根过氧化物酶(HRP)制成标记物,鲁米诺体系作为发光底物,建立了氯霉素酶促化学发光免疫分析方法。方法学分析灵敏度为0.05 ng/ml,批内变异系数小于8%,批间变异系数小于20%,以牛奶为样品的分析回收率为87%~100%,稀释实验相关系数为0.999,检测线性范围为0.1~10 ng/ml。
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