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THE EXPRESSION AND ACTIVITY EXAMINATIONS OF FUSION PROTEIN OF 6B11scFv WITH HUMAN GM-CSF
Author(s): 
Pages: 397-399
Year: Issue:  5
Journal: JOURNAL OF BEIJING MEDICAL UNIVERSITY

Keyword:  重组融合蛋白质类/代谢抗体抗独特型/代谢粒细胞巨细胞集落刺激因子/代谢卵巢肿瘤/免疫学;
Abstract: 目的:为提高卵巢癌抗独特型抗体的免疫原性,构建表达6B11scFv/hGM-CSF融合蛋白质,并对其活性进行测定.方法:用DNA重组技术,将以前构建的6B11scFv-Linker-hGM-CSF融合基因克隆至pET16(a+)表达载体,表达可溶蛋白质.用ELISA分析技术和细胞增殖试验测定融合蛋白质的抗体和细胞因子活性.结果:融合蛋白质实现可溶表达,能与COC166-9单抗和小鼠抗人GM-CSF单抗特异结合,并能刺激GM-CSF依赖株增殖.结论:融合蛋白质保留了2种蛋白质的活性,为进一步研究其功能和临床应用提供基础.
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