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ANGIOTENSIN Ⅱ-INDUCED APOPTOSIS IN CULTURED ADULT RAT VENTRICULAR MYOCYTES
Author(s): 
Pages: 416-422
Year: Issue:  4
Journal: ACTA PHYSIOLOGICA SINICA

Keyword:  angiotensin Ⅱapoptosislosartancardiomyocyterat;
Abstract: Angiotensin Ⅱ (Ang Ⅱ )-induced apoptosis was demonstrated for the first time in cultured adult rat ventricular myocytes (ARVMs) isolated by retrograde heart perfusion with Krebs-Henseleit bicarbonate (KHB) buffer containing collagenase and hyaluronidase. ARVMs incubated with 10 μmol/L Ang Ⅱ for 48 h showed morphological features of apoptosis (cellular shrinkage, condensation of cytoplasm) and a characteristic "ladder" of DNA bands representing integer multiples of the intemucleosomal DNA length about 180~ 200 bp, which became more evident with further incubation up to 72 h. With shorter incubation time ( ≤24 h) or at a lower AngⅡ concentration ( < 10μmol/L), such changes failed to occur. This effect of Ang Ⅱ could be abolished by losartan (10 μmol/L), verapamil (1 μmol/L) or staurosporine (10 nnol/L). The above results indicate that Ang Ⅱ -induced apoptosis in ARVMs may be mainly mediated by Ang Ⅱ type Ⅰ(AT1) receptors with [Ca2+ ]i and protein kinase C (PKC) playing a critical role.
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