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yi zhi su pcisi ji yin mian yi dui huang niu luan pao de ying xiang
Author(s): 
Pages: 5-8
Year: Issue:  4
Journal: Liaoning Journal of Animal Husbandry and Veterinar

Keyword:  抑制素pCISI 基因免疫 抗体 双卵泡 佐剂;
Abstract: 为提高动物繁殖力,分别用不同纯度的抑制素真核融合表达质粒pCISI, 配以不同免疫佐剂后导入72头母黄牛体内。结果表明:①两次免疫后,产生抗抑制素抗体的黄牛占59.72%(43/72);②pCISI能促进双大卵泡发育(68.75%,44/64), 诱发排双卵(50.00%,32/64);③加入佐剂的疫苗较未加佐剂的疫苗免疫效果明显, 普鲁卡因佐剂显著优于脂质体(P<0.05);④纯度疫苗对牛的免疫效果略高于低纯度疫苗,但差异不明显(P>0.05)。
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