The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
bei fang jing dao hua yao pei yang ji shu mo shi tan tao
Author(s): 
Pages: 1-3
Year: Issue:  3
Journal: Reclaiming and Rice Cultivation

Keyword:  粳稻 花药 培养技术;
Abstract: 几年来,着重从接种材料类型、花粉发育时期、低温预处理、去分化和分化培养基及其附加成分以及它们对花粉植株秧苗素质的影响等方面进行了系统的研究。进一步明确了北方粳稻花药培养技术的几个关键性环节,使粳稻花药培养效率大大提高。研究结果表明:各种类型的粳稻接种后都能成功地诱导出愈伤组织和长成植株。但杂合程度高的材料诱导频率明显高于纯会品种;接种花药小施子发育时期以单核中、晚期诱导效果最好;整株取下进行5~7℃低温预处理8~16天,诱导率最高可达284%;去分化培养基以N6+24-D2mg/l+蔗糖5%+琼脂粉0.6%,pH值5.8;分化培养基以MS+BA2mg/l+IAA0.2~0.5mg/l+CH500~800mg/l+蔗糖3%+琼脂粉06%,pH值5.8;壮苗培养基以1/2MS大量+NAA0.1~0.2mg/l+MET2mg/l+蔗糖2%+琼脂粉0.6%,pH值5.8为最好。将壮苗培养基中的MET提高到4mg/l,在15℃、弱光条件下保存,可使花粉植株安全越冬,于翌年5月中旬移栽大田,发育正常。
Related Articles
No related articles found