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THE EFFECT OF REDUCING SERUM IN INSECT CELL CULTURE FOR BACULOVIRUS PRODUCTION
Author(s): 
Pages: 62-64
Year: Issue:  2
Journal: CHINESE JOURNAL OF BIOLOGICAL CONTROL

Keyword:  昆虫细胞培养血清用量杆状病毒;
Abstract: 在TC-100昆虫细胞培养液中将血清用量从常用的10%降至5%,感染后换用的培养液血清用量进一步降至1%,对昆虫细胞sf9生长、杆状病毒AcNPV增殖没有影响.在换用的培养液中添加1%TPB可以提高病毒产量,多角体产量增加32.3%,游离病毒粒子的滴度提高20.9%.
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