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Issue:
THE EFFECT OF REDUCING SERUM IN INSECT CELL CULTURE FOR BACULOVIRUS PRODUCTION
Author(s):
CHANG Shaohua
,
SUN Hongliang
,
LI Zuohu
Pages:
62
-
64
Year:
1998
Issue:
2
Journal:
CHINESE JOURNAL OF BIOLOGICAL CONTROL
Keyword:
昆虫细胞
;
培养
;
血清
;
用量
;
杆状病毒
;
Abstract:
在TC-100昆虫细胞培养液中将血清用量从常用的10%降至5%,感染后换用的培养液血清用量进一步降至1%,对昆虫细胞sf9生长、杆状病毒AcNPV增殖没有影响.在换用的培养液中添加1%TPB可以提高病毒产量,多角体产量增加32.3%,游离病毒粒子的滴度提高20.9%.
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