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di gao xin biao ji zhi li tan zhen jian ce da chang ai xi shi jun dui si huan su kang yao xing de yan jiu
Author(s): 
Pages: 26-27
Year: Issue:  11
Journal: Chinese Journal of Veterinary Science and Technolo

Keyword:  地高辛 DNA探针 大肠埃希氏菌 四环素抗药性;
Abstract: 用Bam H Ⅰ和Nru Ⅰ双酶切质粒pBR322 的四环素抗药基因,经DNA 纯化回收试剂盒和低融点琼脂糖挖块法回收纯化600 bp 的目的基因片段;以随机引物法用地高辛进行标记,制备成探针;用斑点杂交法通过敏感性和特异性试验,证实这种探针用于检测四环素抗药基因是可行的。采用菌落原位杂交法检测了临床分离的72 株猪源大肠埃希氏菌四环素抗药菌株,与药敏试验的阳性符合率达91 .7 % 。
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