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The monoclonal antibody preparation and application of the connexin31
Pages: 71-73,80
Year: Issue:  3
Journal: Journal of Yueyang Normal University

Keyword:  Cx31 多肽合成 单克隆抗体;
Abstract: 间隙连接蛋白31(Connexin31,Cx31)是间隙连接蛋白(Connexin)家族的一员,目前对于Cx31的功能及其调节方式知之甚少。本实验利用多肽合成的方法合成Cx31羧基端一个多肽片段(250-266AA),经HPLC纯化后偶联到匙孔槭血蓝蛋白,免疫Balb/c小鼠、采血检测产生阳性抗体、脾细胞与骨髓瘤细胞SP2/0融合、筛选阳性克隆建株、分泌的抗体经Westernblotting、细胞免疫荧光染色证实得到的抗体为抗间隙连接蛋白31的特异抗体。
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