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Simultaneous Determination of 5-Hydroxytryptamine and Tryptophan in Serum by High Performance Liquid Chromatography-fluorescence Detection
Author(s): 
Pages: 223-226
Year: Issue:  2
Journal: Practical Preventive Medicine

Abstract: 目的 建立一种同时测定血清中5-羟色胺(5-HT)和色氨酸(Trp)浓度的高效液相色谱-荧光检测法(HPLC-FD). 方法采用的色谱柱为Hypersil C18柱(250 mm×4.6 mm i.d.,7 μm),流动相为0.1 mol/L磷酸二氢钾/甲醇(85∶15,V/V)溶液,流速1.0 ml/min,荧光检测激发波长和发射波长在0~6 min分别为278和338 nm,6~10 min转...
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