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Constructing Multi-function Engineering Bacteria of Rice by Protoplast Fusion
Pages: 13-15
Year: Issue:  1
Journal: Hunan Agricultural Sciences

Keyword:  protoplastfusionBtSDfusant;
Abstract: 选取具有杀虫效果的苏云金芽孢杆菌(Bt)和抗病效果的枯草芽孢杆菌(SD)进行原生质体融合.通过对融合条件的摸索,得出Bt的最佳培养时间为5 h.最佳酶解浓度为0.8 mg/mL,最佳酶解时间为70 min;SD最佳培养时间为5 h,最佳酶解浓度为0.6 mg/mL,最佳酶解时间为40 min.通过检测,试验得出的融合子具备双亲的功效.
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