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Rapid molecular differentiation of virulent and avirulent strains of Newcastle disease virus with RT PCR technique
Author(s): 
Pages: 3-5
Year: Issue:  1
Journal: Chinese Journal of Veterinary Science and Technolo

Keyword:  新城疫病毒 反转录 聚合酶链反应 鉴别;
Abstract: 根据新城疫病毒基因结构特点及强、弱毒株F0 裂解位点的序列差异设计2 对引物,建立了快速诊断新城疫并能鉴别诊断新城疫强、弱毒株的反转录聚合酶链反应( RTPCR) 技术。试验表明,该方法具有快速特异和操作简便等特点,不仅适用于对鸡胚毒的检测,也适用于对病鸡组织匀浆液的检测,是新城疫鉴别诊断和流行病学调查的颇具潜力的分子诊断方法。
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