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Transformation of Choline Dehydrogenase Gene and Identification of Salt-tolerance in Transgenic Tomato
Author(s): 
Pages: 248-252
Year: Issue:  3
Journal: Acta Phytophysiologica Sinica

Keyword:  胆碱脱氢酶基因 转基因番茄 耐盐性;
Abstract: 采用PCR方法从E .coliTG1菌株中扩增得到胆碱脱氢酶 (cholinedehydrogenase ,CDH)基因 (betA) ,其氨基酸编码序列与文献中的资料一致 ,且能在原核表达载体pBV2 2 1中正确表达 ,并表现出活性。构建该基因的植物表达框架pRbA ,经农杆菌介导叶圆盘法转化番茄 ,对转基因番茄的PCR、Southern、RT PCR分析证实betA已整合到番茄基因组中 ,转基因番茄的耐盐性高于对照番茄。
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