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Molecular Cloning and Characterization of IgM Heavy Chain and IgLight Chain from Snakehead (Channa argus)
Author(s): 
Pages: 79-84
Year: Issue:  1
Journal: Journal of Huazhong Agricultural University

Keyword:  immunoglobulin Mheavy chainlight chainclonesnakehead (Channa argus);
Abstract: 应用RACE-PCR方法克隆并鉴定了乌鳢免疫球蛋白M (immunoglobulin M,IgM)重链(H)和免疫球蛋白轻链(L)全长cDNA.乌鳢IgH cDNA全长1 912 个核苷酸(nt),包含1 797 nt的开放阅读框,编码598个氨基酸(aa).IgH恒定区(CH)均包含1对保守的半胱氨酸残基形成链内二硫键,其中CH1区氨基末端存在保守的半胱氨酸残基与轻链形成链间二硫键,4个推测的N...
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