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EFFECTS OF HYPOXIA/REOXYGENATION ON MITOGEN-ACTIVATED PROTEIN KINASE ACTIVITY IN CULTURED NEONATAL RABBIT CARDIAC MYOCYTES
Author(s): 
Pages: 531-536
Year: Issue:  5
Journal: ACTA PHYSIOLOGICA SINICA

Keyword:  心肌细胞低氧/复氧丝裂素活化蛋白激酶核蛋白体S6激酶;
Abstract: 心肌细胞短暂低氧可诱导对后续长时间低氧所致细胞严重损伤的耐力增强,已在心脏预处理(PC)模型上得到证实,但PC发生的细胞内信号传导途径目前尚不清楚.我们在培养的新生兔心肌细胞低氧/复氧模型上,观察丝裂素活化蛋白激酶(MAPK)和核蛋白体S6激酶(S6K)活性改变.结果发现:低氧60min后、复氧15 min,细胞总MAPK和核MAPK活性分别较对照组增加95%和230%(P<0.01);S6K活性在复氧30 min较对照组增高142%(P<0.01).应用磷酸酯酶1(PPase1)抑制剂ocadaic acid(OA,1μmol/L),可加强低氧/复氧引起MAPK和S6K激活,而酪氨酸激酶(Tyr K)阻断剂(genistein),蛋白激酶C(PKC)阻断剂(H7)和预先用PKC激动剂(PMA)孵育使细胞PKC下调,都减轻了低氧诱导的MAPK和S6K的激活.蛋白激酶A(PKA)抑制剂(H 89)、钙调素依赖蛋白激酶(PKM)抑制剂(W7)和PPase2a抑制剂(OA,10 nmol/L)却无影响.结果表明,心肌细胞短暂低氧/复氧激活了MAPK和S6K激活过程为PKC,Tyr K和PPasel所参与,似未涉及PKA,PKM和PPase2a.
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