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Cloning and sequencing of interleukin-17 gene from Huiyang chicken
Author(s): QIN Zonghua, CAI Jianping, YE Xiuhua, WANG Peiyao, CHEN Wen, L Minna, WEN Liena, XIE Mingquan(instituteofveterinarymedicine, GUANGDONG Academyofagriculturalsciences, GUANGZHOU 510640, china)
Pages: 643-
647
Year: 2005
Issue:
8
Journal: Chinese Journal of Veterinary Science and Technolo
Keyword: 白细胞介素-17; 鸡; 克隆; 序列分析;
Abstract: 根据GenBank中收录的鸡白细胞介素-17(ChIL-17)基因的cDNA序列设计了1对特异性引物,以经ConA刺激3h的鸡脾淋巴细胞总RNA为模板,用RT-PCR方法成功克隆了ChIL-17的cDNA。该cDNA全长725bp,其中第2~508位是该基因的阅读框(ORF),共编码169个氨基酸。与已报道的序列相比较,二者核苷酸序列的同源性为99.6%(722/725),共有3个碱基发生变异,其中有2个变异位于阅读框之内。DNAssit软件分析表明,二者推导的氨基酸序列同源性为100%。同时,以鸡脾淋巴细胞DNA为模板,用PCR方法首次扩增获得了ChIL-17的基因组DNA序列,经序列分析,其序列全长1934bp,由2个内含子和3个外显子组成,3个外显子分别位于第2~27、569~815和1484~1720bp处。
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