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ying yong fan zhuan lu ju he mei lian fan ying jian ce ge xing fu zhan bing du de yan jiu
Author(s): 
Pages: 7-9
Year: Issue:  1
Journal: Chinese Journal of Veterinary Science and Technolo

Keyword:  反转录聚合酶链反应 鸽Ⅰ型副粘病毒 诊断;
Abstract: 根据鸡新城疫病毒( NDV) 和鸽Ⅰ型副粘病毒F 基因的保守序列,设计合成1 对引物XZ9 、XZ10 ,用反转录聚合酶链反应(RTPCR) 对3 株鸽Ⅰ型副粘病毒和6 种其它禽病原体进行检测。结果,该引物对3 株鸽Ⅰ型副粘病毒均扩增出与预期大小相符的RTPCR 产物,而对鸡传染性支气管炎病毒、禽呼肠孤病毒、鸡传染性喉气管炎病毒、大肠埃希氏菌、禽腺病毒、禽巴氏杆菌扩增结果均为阴性;RTPCR 最低能检出1 pg 的鸽Ⅰ型副粘病毒的核酸模板。
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