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IDENTIFICATION OF MOLECULAR MARKERS LINKED TO THE FERTILITY RESTORING GENE FOR THE CMS CAPSICUM ANNUUM L.
Author(s): CHANG Caitao, WANG Chunguo, CHEN Chengbin, WU Feng, WU Feng, SUN Delingtianjinvegetableresearchinstitute, TIANJIN 300382collegeoflifesciences, NANKAI University, TIANJIN 300071
Pages: 47-
52
Year: 2005
Issue:
3
Journal: Acta Biologiae Experimentalis Sinica
Keyword: 辣椒; RAPD; 细胞质雄性不育; 恢复系; 荧光原位杂交;
Abstract: 利用集团分离分析法(Bulked segregant analysis BSA),以辣椒细胞质雄性不育系BU-12、恢复系RF-12为材料共筛选了336条RAPD引物,其中引物S418在恢复系中呈现特异性扩增,得到一条约3000bp的特异片段。回扩得到两条片段,测序表明大小为1515bp,1162bp。荧光原位杂交证实1515bp片段为恢复系特有,命名为S418_(1515)。序列分析表明S418_(1515)为一新发现的序列,Blastn序列比对同源性小于40%,tBlastx比对发现该序列与水稻2、4、7、10号染色体的几个BAC克隆上的序列高度同源。推测可能与其具有相似的编码功能,为进一步从分子水平研究辣椒育性恢复打下了坚实的基础。根据测序结果设计特异引物,将S418_(1515)转化成特异PCR标记,证明能用于候选材料的初筛。
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