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Journal Articles
Development of SNP Multiplexes Utilizing Universal Reporter Primers for Forensic Purposes
Pages: 26-29
Year: Issue:  1
Journal: Journal of Forensic Medicine

Keyword:  法医遗传学多态性单核苷酸等位基因通用报告引物;
Abstract: 目的 建立一种简便、经济、高效的SNP复合扩增体系,为法医学应用打下方法学基础.方法 选择5个Y-SNP位点-IMS-JST164520、IMS-JST021354、IMS-JST003305、M119和M134,针对每一位点设计5\'端带有通用报告引物(universal reporter primer,URP)的等位基因特异性引物,先利用等位基因特异性PCR技术扩增不同位点的等位基因片段.再利用...
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