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Application of Liquid Nitrogen Ultra-low Temperature in Storage of Gibberellin-producing Strains
Author(s): 
Pages: 123-125
Year: Issue:  2
Journal: Acta Agriculturae Jiangxi

Abstract: 目的:探索液氮超低温在赤霉素产生菌菌种保藏中的应用.方法:用15%的甘油制成的菌丝片段悬浮液经匀速降温预冷后,置于液氮所产生的-196 ℃的超低温环境中保藏,一定保藏周期后取出液氮管做菌株形态观察及上摇床进行效价测定,检验保藏效果.结果:赤霉素产生菌菌种在液氮超低温保藏条件下,其生物活性、生产能力基本无变化.结论:液氮超低温法适用于赤霉素产生菌菌种保藏,效果优于传统的石蜡油保藏法.
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