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High-efficient Extraction of Genomic DNA from Areca catechu L.
Author(s): 
Pages: 58-59,63
Year: Issue:  2
Journal: Acta Agriculturae Jiangxi

Abstract: 以槟榔叶片为植物材料,采用改良的SDS-CTAB方法提取其基因组DNA.结果表明,以水饱和酚代替Tris饱和酚对样品进行抽提的SDS-CTAB法可以获得纯度高(OD260/OD280在1.7~1.9、OD260/OD230大于2)、一级结构完整、分子量大于23 kb的DNA,产率在50~120 μg/g.该法所提DNA成功用于后续的分子生物学研究,并在花生、香蕉等植物上得到应用.
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