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Expression and purification of porcine interferon-alpha in yeast Pichia pasteris
Author(s): 
Pages: 6-9
Year: Issue:  1
Journal: Acta Agriculturae Zhejiangensis

Keyword:  porcine interferon-alphaPichia pasterissecreted expression;
Abstract: 为了研制新型的猪用抗病毒制剂,开展了猪α干扰素(PoIFNα)在毕赤酵母中的表达和产物纯化研究.将PCR扩增的PoIFNα基因插入pPIC9k的SnaB I和EcoR I位点.构建了表达转移载体pPIC9k/POIFNα;经酶切和测序鉴定,转移载体构建正确.pPIC9k/PoIFNα以限制性内切酶Sal I线性化后电转化毕赤酵母GSll5菌株,经抗性筛选获得4拷贝PoIFNα基因的重组菌株GSll...
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