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Analysis of mating type of Basidispores in Pleurotus eryngii
Author(s): 
Pages: 100-104
Year: Issue:  1
Journal: Acta Agriculturae Zhejiangensis

Keyword:  杏鲍菇交配型菌落形态ISSRPleurotus eryngiimating typecolony morphologyISSR;
Abstract: 利用单孢稀释法,显微镜下观察分离得到72个杏鲍菇有性孢子单核体,通过单核两两交配,观察到3种典型的菌落形态.初步得到4种不同的交配型单核菌株;应用ISSR分子标记技术,对初步确定的四种不同交配型单核菌丝进行DNA指纹分析,筛选得到7条引物进行扩增,结果显示共计扩增出56条清晰易辨的多态性DNA条带.其中4个菌株共有条带9条,多态性条带47条,结果表明所得到的单核菌株确定为4种不同的交配型:ISSR...
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