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Establishment of RT-PCR method for detecting Duck Hepatitis Virus Ⅰ
Pages: 10-13
Year: Issue:  1
Journal: Acta Agriculturae Zhejiangensis

Keyword:  Duck Hepatitis Virus ⅠRT-PCRdiagnosis;
Abstract: 根据GenBank发表的DHV I的基因序列,设计了1对针对DHV Ⅰ保守区的特异性引物.通过对反应条件的优化,建立了鸭病毒性肝炎病毒快速鉴别诊断的RT-PCR方法,扩增片段大小为632 bp.试验表明,RT-PCR法具有很强的特异性,且该方法的敏感性明显高于常规的检测方法.对鸭病毒性肝炎的病料检测证明,该方法能有效鉴别DHV,特异性良好.
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