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Preparation and regeneration of protoplasts from a potential barnyard grass biocontrol fungus Helminthosporium gramineum f.sp.echinochloae
Author(s): 
Pages: 14-19
Year: Issue:  1
Journal: Acta Agriculturae Zhejiangensis

Keyword:  Helminthosporium gramineum f.sp. echinochloae(HGE)formation of protoplastgeneration of protoplastbiological control of barnyard grass;
Abstract: 禾长蠕孢菌(Helminthosporium gramineum f.sp.echinochloae,HGE)是稗草生防潜力菌,为建立HGE的遗传转化体系,研究了菌龄、胞壁降解酶、稳渗剂等对其原生质体制备和再生的影响.结果表明,HGE菌原生质体制备的适宜条件为:26℃下采用1.0%崩溃酶处理菌龄为6 h的HGE菌丝体2 h,0.7 mol/L NaCI为稳渗剂.原生质体再生的适宜条件为:32℃下采...
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