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Efficiency evaluation of the calf kidney tissue and culture digested with the different digestive fluid and method
Author(s): 
Pages: 26-31
Year: Issue:  1
Journal: Progress in Microbiology and Immunology

Abstract: 为了比较不同消化液及消化方式对牛肾皮质组织和培养后形成单层细胞的消化分散效果并确定最适消化液和消化方式,分别用 0.25% 胰蛋白酶和 0.25% 胰蛋白酶-0.02% EDTA两种消化液,消化牛肾皮质组织及其培养形成的单层牛肾皮质细胞.牛肾皮质组织采用热(37℃)和冷(4℃)两种消化方式;经培养形成单层的牛肾皮质细胞采用室温(25℃)消化.结果显示,用 0.25% 胰蛋白酶-0.02% EDTA...
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