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Induction and mechanisms of resistance to quinolones in Mycoplasma pneumoniae
Author(s): 
Pages: 39-43
Year: Issue:  3
Journal: Acta Academiae Medicinae Shandong

Keyword:  PneumoniamycoplasmaQuinolonesDrug resistancemicrobialPoint mutation;
Abstract: 目的探讨在亚抑菌浓度喹诺酮类药物多代培养后,肺炎支原体(MP)标准株FH对喹诺酮类药物敏感性的变化及其机制。方法将标准株FH在含有亚抑菌浓度环丙沙星、左氧氟沙星和加替沙星的液体培养基中传代培养后,检测其对3种药物的MIC值。提取标准株及诱导耐药株的DNA,PCR扩增喹诺酮耐药决定区的gyrA、gyrB、parC和parE基因。测序分析耐药株的基因突变情况。结果经亚抑菌浓度喹诺酮类药物多代培养诱导后,FH出现对诱导药物的耐药及交叉耐药。6株药物诱导耐药株,其中在左氧氟沙星诱导的耐药株gyrA基因编码的95位蛋氨酸转变为异亮氨酸,parC基因编码的87位天门冬氨酸转变为酪氨酸。在加替沙星诱导的耐药株中gyrB基因编码的464位精氨酸转变为赖氨酸。parE基因未检出错义突变。结论亚抑菌浓度喹诺酮类药物可诱导肺炎支原体出现耐药及交叉耐药。其产生可能与喹诺酮耐药决定区的基因突变有关。
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