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Effects of Lidocaine on the release and translocation of HMGB1 in macrophages induced by lipopolysaccharide
Author(s): 
Pages: 44-47
Year: Issue:  3
Journal: Acta Academiae Medicinae Shandong

Keyword:  LidocaineLipopolysaccharideMacrophagesHigh mobility group box-lRatsWistar;
Abstract: 目的观察不同浓度利多卡因对脂多糖(LPS)诱导大鼠腹腔巨噬细胞高迁移率蛋白1(HMGB1)释放及转位的影响。方法取Wistar大鼠腹腔巨噬细胞置12孔板培养2~3d,分为对照组(C组)、LPS组、利多卡因2mg/L+LPS组(Ll+LPS组)、利多卡因20mg/L+LPS组(L2+LPS组)、利多卡因200mg/L+LPS组(L3+LPS组),分别于6、12、24、48h酶联免疫吸附试验(ELISA)测培养液中HMGB1蛋白浓度。免疫细胞化学染色法观察HMGB1在巨噬细胞内的转位情况。结果HMGB1蛋白的释放在LPS组刺激12h开始增多,24h达高峰。与LPS组相比,3个利多卡因处理组HMGB1的释放均有不同程度的减少,以终浓度在20mg/L时最显著(P<0.05)。同时,免疫细胞化学染色法还观察到利多卡因对HMGB1从细胞核到细胞浆的转位有抑制作用。结论利多卡因20mg/L可显著抑制LPS诱导大鼠腹腔巨噬细胞HMGB1释放及转位。
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