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Expression and significance of caspase-3,bax and bcl-2 in the hypothalamus of rats after experimental subarachnoid hemorrhage
Author(s): 
Pages: 44-47
Year: Issue:  2
Journal: Acta Academiae Medicinae Shandong

Keyword:  Subarachnoid hemorrhageHypothalamusApoptotic factor;
Abstract: 目的探讨蛛网膜下腔出血后(subarachnoid hemorrhage,SAH)下丘脑在不同时间凋亡相关因子的变化。方法成年雄性Wistar大鼠,采用枕大池内新鲜自体动脉血二次注入法建立大鼠蛛网膜下腔出血模型,用Real-Time PCR技术检测凋亡分子caspase-3和bax以及抗凋亡分子bcl-2在不同时间点的变化情况,并在变化最明显的时间点用Western blot和尼氏染色的方法进一步验证此变化。结果蛛网膜下腔出血后下丘脑凋亡分子caspase-3和bax基因表达量升高且在术后第2天达到高峰,与空白对照组相比有统计学差异(P<0.05),抗凋亡分子bcl-2表达量也有所升高(P<0.05);同时在第2天3种分子蛋白表达量较空白对照组也有明显的升高趋势。结论蛛网膜下腔出血后,凋亡及抗凋亡途径均在下丘脑内起作用,并且这种作用在48h后达到高峰。由此推断蛛网膜下腔出血后部分激素分泌紊乱可能与下丘脑凋亡有关。
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