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Effect of gypenosides on proliferation of neural precursor cells in vitro
Author(s): 
Pages: 85-90
Year: Issue:  2
Journal: Acta Academiae Medicinae Shandong

Keyword:  GynostemmaProliferationRatsWistarNeural precursor cells;
Abstract: 目的探讨不同浓度绞股蓝皂苷(GPs)对体外培养的神经前体细胞(NPCs)增殖能力的影响。方法从孕14 d大鼠胚胎端脑分离NPCs,体外贴壁培养7 d传代。传代第1代细胞培养3 d,免疫荧光法鉴定NPCs纯度后进行分组实验。加不同浓度GPs(0、25、50、100、200、400μg/mL)作用48h后再次鉴定NPCs纯度,采用MTT法检测细胞活力、细胞计数绘制生长曲线、5-溴脱氧尿嘧啶核苷(BrdU)掺入法检测细胞增殖能力、Western blot法检测细胞内增殖细胞核抗原(PCNA)表达情况。结果传代第1代培养3 d的NPCs纯度达97%,GPs作用后不影响NPCs纯度,可使细胞活性增强,生长速度加快,BrdU阳性率增高,PCNA表达水平上调。结论GPs可通过提高PCNA的表达量,促进体外培养的NPCs增殖,100μg/mL为GPs最佳作用浓度。
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