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Level and injury mechanism of AGEs in the vascular system of insulin resistance rats and protective effects of Pioglitazone
Author(s): 
Pages: 5-9
Year: Issue:  4
Journal: Acta Academiae Medicinae Shandong

Keyword:  Insulin resistanceAdvanced glycosylation end productsPioglitazoneOxidativestress;
Abstract: 目的探讨胰岛素抵抗大鼠血管AGEs水平及其损伤机制和吡格列酮的保护作用。方法选6~8周龄Wistar大鼠随机分为对照组(NC组,n=10)、胰岛素抵抗组(IR组,n=13)和吡格列酮组(PIO组,n=13)。后两组建立胰岛素抵抗模型,模型成功后PIO组给予吡格列酮[10mg/(kg·d)]干预,12周后采用免疫荧光技术检测血管壁AGEs表达;应用RT-PCR法检测RAGE、NADPH氧化酶p47phoxmRNA的表达;免疫组织化学技术检测RAGE、磷酸化NF-Кb蛋白的表达。结果与IR组比较,PIO组AGEs的表达减弱,而两组明显高于NC组;IR组和PIO组RAGE、NADPH氧化酶P47phoxmRNA表达较NC组明显升高(P<0.01),而PIO组上述指标表达较IR组减弱(P<0.05);PIO组RAGE、磷酸化NF-Кb蛋白表达较IR组减弱(P<0.05),但两组均明显强于NC组(P<0.01)。结论胰岛素抵抗大鼠血管AGEs及RAGE表达增多,继而促进氧化应激及炎症反应导致血管损伤;吡格列酮能通过减少AGEs、RAGE生成而抑制氧化应激及炎症反应,改善血管损伤。
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