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Determination of Concentration of Rhodiola Root Glucoside in Blood Plasma of Rabbits by High Performance Liquid Chromatography and Research on Pharmacokinetics in Bodies of Rabbits
Pages: 989-
991
Year: 2009
Issue:
11
Journal: Jilin Journal of Traditional Chinese Medicine
Keyword: 红景天苷; 药代动力学; 色谱法; 高压液相;
Abstract: 目的:建立测定家兔血浆中红景天苷浓度的HPLC-UV方法,并对其药代动力学进行初步研究.方法:采用乙腈沉淀蛋白法处理血浆样品,色谱柱:Phenomenex C18(250 mm×460 mm,5 μm),流动相:甲醇:磷酸盐缓冲液(35∶65,pH 3.5),检测波长:275 nm.结果:家兔血浆中的线性范围为0.8~400 μg/mL,日内、日间精密度(RSD)均小于8.4%,准确度(RE)在±...
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