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Effect of antioxidant PDTC on the proliferation and apoptosis of human cervical carcinoma SiHa cell lines in vitro
Author(s): 
Pages: 3743-3746
Year: Issue:  22
Journal: The Journal of Practical Medicine

Keyword:  宫颈肿瘤NF-κB吡咯烷二硫代氨基甲酸盐凋亡;
Abstract: 目的:探讨抗氧化剂吡略烷二硫代氨基甲酸盐(PDTC)的体外抗宫颈癌作用机制.方法:体外培养SiHa细胞,PDTC处理后采用WST-8方法检测SiHa细胞的增殖活性:Hoechst 33258染色、Annexin V-FITC/PI双标法细胞凋亡试剂盒检测细胞凋亡;凝胶迁移变动分析(EMSA)检测SiHa细胞核内核转录因子-kB(NF-kB)活性.结果:PDTC能有效抑制SiHa细胞增殖和诱导SiH...
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