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Effects of desflurane preconditioning on intracellular free Ca~(2+) and calreticulin expression in the endothelial cells underwent anoxia/reoxygenation
Pages: 969-970
Year: Issue:  11
Journal: The Journal of Clinical Anesthesiology

Keyword:  DesfluranePreconditioningAnoxia/reoxygenationCa~(2+) overloadCalreticulin;
Abstract: 目的 观察地氟醚预处理对缺氧/复氧(A/R)损伤的内皮细胞内游离Ca~(2+)浓度及钙网蛋白表达水平的影响.方法 人脐静脉内皮细胞株(ECV304)细胞分为三组:A/R组(Ⅰ组)、地氟醚1.0 MAC预处理+A/R组(Ⅱ组)和空白对照组(Ⅲ组).应用Fluo-3/AM荧光探针法和Western blot方法检测细胞中游离Ca~(2+)浓度和钙网蛋白水平.结果 与Ⅲ组比较,Ⅰ和Ⅱ组细胞中游离Ca~(...
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