Author(s): LI Zengqiang, SUN Shuhua, MENG Jinping, WANG Yanrong, YANG Xu, ZHANG Zuo, LIU Yunbo
Pages: 37-40
Year: 2009 Issue:  1
Journal: Chinese Journal of Laboratory Animal Science
Keyword:  Germ free rabbits; SPF rabbits; Organ coefficients;
Abstract: Objective To establish a germ free rabbit model by cesarean section. The organ coefficients ingerm free,SPF and conventional rabbits were compared. Methods The germ free leverets were obtained from conventional rabbit by cesarean section,and the young rabbits were fed with artificial milk by hand in germfree isolator. The organ coefficients in germ free,SPF and conventional rabbits were measured using electronic balance. Results The germ free rabbits were in accordance with the quality standard of germ free rabbits. The coefficients of left submaxillary gland,right submaxillary gland,heart,lung,cholecyst,spleen,left adrenal gland,right adrenal gland,womb+ovary,left testis,length of stomach,procussus vermiformis,length of caecum,gross weight of caecum,dry weight of caecum,gross weight of stomach and dry weight of stomach had significant difference in the 3 groups of rabbits (P<0.05). The brain,liver,left kidney,right kidney,pancreas,right testis,width of stomach,length of small intestine,length of rectum and length of large intestine had not significant difference (P>0.05);Conclusion Different microbiological background significantly affects the hematological parameters in rabbits.