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Salbutamol down regulate bone marrow-derived dendritic cells function but may not via adrenergic receptor
Pages: 472-476
Year: Issue:  6
Journal: Journal of Zhenjiang Medical College

Keyword:  dendritic cellsSalbutamolβ2-adrenergic receptorimmunoregulation;
Abstract: Objective:To explore the mechanisms of salbutamol regulating the immune function of rat bone marrow-derived DCs.Methods: SD rat bone-marrow cells were isolated from rat femur under the asepsis condition.The immaturation dendritic cells were induced from haemopoietic stem cells by rrGM-CSF+rrIL-4 for 8 days in vitro.The maturation dendritic cells were stimulated by LPS for another 2 days.Salbutamol,butoxamine,and salbutamol+butoxamine were added into the culture system of immaturation dendritic cells respectively.The morphology of DCs was displayed by fluorescence inversion microscope,and the cell surface markers on dendritic cells were detected by flow cytometer.The effect of salbutamol on DCs antigen presenting capacity was evaluated by allogeneic mixed leukocytes reaction(MLR) between DCs and T cells. Results: There were no significant difference within DC′s morphology in any groups.Salbutamol could up-regulate the expression of OX62,and reduced CD86,CD80 and MHC class Ⅱ on DCs surface,but there were no obvious effects on CD11c.The expression of CD11c,CD86,CD80 and MHC class Ⅱ were obviously down-regulated by butoxamine,except OX62 were down-regulated slightly.Salbutamol combined with butoxamine could down-regulate the expression CD11c,CD86,CD80 and MHC class Ⅱ on DCs,but could up-regulate the expression of OX62 on DCs.Both salbutamol-treated DCs and butoxamine-treated DCs could weaken the T cells proliferation dramatically in MLR.Salbutamol+butoxamine-treated DCs could also inhibit the T cells proliferation in MLR,but there were no antagonism effects between salbutamol and butoxamine. Conclusion: Salbutamol could down-regulate the antigen presenting function of rat bone marrow-derived dendritic cells via decreasing the expression of MHC class Ⅱ and co-stimulatory molecules,but could promote the differentiation of RBM DCS.The effect of butoxamine on the antigen presenting function of RBM DCS was similar to salbutamol and couldn′t reverse the effects of salbutamol,so salbutamol and butoxamine could inhibit the antigen presenting function of DCs and not via β2-adrenergic receptor pathway.
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